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1 instruments and reagents

Shuanghuanglian injection determination of baicalin methods include HPLC,9jSITory Burch Amanda Leather Hobo - Purses, Designer Handbags and Reviews at The Purse Page, TLC, UV spectrophotometry,ghd australia, etc., high performance liquid chromatography with high separation efficiency, fast and the precise characteristics, suitable for Chinese medicines and the complexity of the diversity in the quality control on Shuanghuanglian applications are up to. This determination by optimizing the chromatographic conditions the content of baicalin to Shuanghuanglian injection for further study of chlorogenic acid and other ingredients to lay foundation for Shuanghuanglian injection technology research and quality control theory.

2.2.2 Preparation of the test solution with 0.1 ml pipette from the lessons Shuanghuanglian precision injection to 10 ml 0.05 ml volumetric flask with methanol (AR ) to the mark, ultrasound allows the dissolved 10 min. 0.45 μm membrane filter and then the lotion to soak 3 h with chromic acid and dried well in 10 ml colorimetric tube, tightening the plug.

Abstract aims to establish a high performance liquid chromatography (HPLC) method for the determination Shuanghuanglian injection method in the component content. Methods of methanol - water (10% phosphoric acid to adjust pH 2.7) as mobile phase, flow rate 1.0 ml / min, detection wavelength was 326 nm, in Diamonsil (diamonds) C18 (5 μm, 4.6 mm × 150 mm) chromatography column elution, external standard baicalin content can be determined. The results of baicalin in the 5.1 ~ 81.6 μg / ml range was a good linear relationship, r = 0.999 8, the average recovery was 100.34%, RSD = 0.82% (n = 5). Conclusion The method is simple, sensitive,Tory Burch Shoes 0aPbsale tory burch Charm Brown, reproducible, accurate,Tory Burch Reva Sale, high precision, good linearity, and higher recovery rate, measured results show that within 12 h of baicalin and stability, suitable for quality control of Shuanghuanglian injection.

Key words baicalin Shuanghuanglian injection high performance liquid chromatography

1.2 Reagents Shuanghuanglian injection (Lot No. Z23021166 Pharmaceutical Co., Ltd. Heilongjiang Ussuri Jia); baicalin chemical standard (batch number 110715-200514, China Pharmaceutical Biological Products); chromatography methanol (Tianjin Chemical Reagent Factory Mao); analytical grade methanol (Tianjin Chemical Reagent Factory in North Tian Yi); phosphate (Baoding, Hebei Province Chemical Reagent Factory).

2.2 Preparation of solution

1.1 Agilent 1100 HPLC instrument (Agilent, USA); TU-1800 UV-visible spectrophotometer (Beijing General Instrument & P analysis of a limited liability company); KQ-100 ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); electronic balance (Shanghai Jing Branch balance); to China SHZ-D (Ⅲ) with water circulation pump (British Yu Gongyi City, Henan Province to the Chinese Instrument); Diamonsil (Diamond ) C18 column (5μm, 4.6 mm × 150 mm Cat.no: 9990 Ser.no: 8011571 Dikma Technologies).

2.3 linear relationship between the concentration of study precise amount of 0.102 mg / ml baicalin 0.5,1,2,scarpe mbt,4,6,8 ml standard stock solution to 10 ml capacity, respectively, flask with methanol (AR) to the mark, the preparation of the concentration of 5.1,10.2,20.4,40.8,61.2,81.6 μg / ml after a series of standard solution, and then 0.45 μm membrane filter to the use of chromium After 3 h immersion pickling good six 10 ml dried colorimetric tube, tightening the plug. The chromatographic conditions were determined by each concentration of standard solution of baicalin measured 3 times. According to the average of 3 times the peak area, find the standard curve of baicalin. The results shown in Table 1, baicalin in 5.1 ~ 81.6 μg / ml concentration range was linear. The regression equation was Y = 41.409X -132.35,Tory Burch Boots, r = 0.999 8.

2.2.1 Preparation of standard solution of baicalin accurately weighed standard 0.005 100 g to 50 ml flask, methanol (AR) to the mark, from ultrasound The completely dissolved. Preparation of a concentration of 0.102 mg / ml stock solution in the refrigerator, cold storage.

Shuanghuanglian injection developed in recent years, Chinese medicine preparations, mainly used in clinical acute upper respiratory tract infection, acute bronchitis, acute tonsillitis, pneumonia and acute urinary tract infection, and its effective and safe, the application is wide. Shuanghuanglian injection by the honeysuckle,Tory Burch outlet, Scutellaria, Forsythia 3 extract made from herbs,Tory Burch Shoes 3aDVBlog » vibram five finger sho, the main component of baicalin, chlorogenic acid, Phillyrin [1]. Pharmacological studies have demonstrated that baicalin has antibacterial, heat, blood pressure, sedative, diuretic, choleretic, anti-inflammatory, anti-allergy, the role of detoxification; chlorogenic acid with antibacterial anti-inflammatory,mbt italia, heat the role of detoxification; and Phillyrin is a strong antibacterial, antiviral activity.

2 Methods and results

2.1 Chromatographic conditions Diamonsil (diamonds) C18 column (5 μm, 4.6 mm × 150 mm), mobile phase A-methanol (HPLC grade): B-distilled water (50:50 ), flow rate 1.0 ml / min, detection wavelength was 326 nm,Tory Burch Handbags, injection volume 20 μl. The chromatographic separation shown in Figure 1 and 2.

Determination of Shuanghuanglian injection method in the analysis of baicalin

Of: Di Li Duan Li River to Di Wu Hua Tong Guorui
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